Heavy Metal Test Science
Dr. Joachim Leman
Medical Laboratory Expert –Toxicologist Germany
Heavy Metal Test (HMST) In the eyes of a chemist, this test is composed of two essential chemical steps. The first one
is a complex forming reaction between metal ions and Dithizone at the phase border separating aqueous solution
and Testsol-phase; the second one is an extraction of the performed complex into the organic layer. The two
essential steps as well as the preceding one and the evaluation procedures are very common processes in
analytical chemistry and form part of many other determination methods used in clinical
chemistry.
Dithizone (1,5-Diphenylthiocarbazone) is a current reagent since
it was introduced in trace-analytical chemistry in 1925. It is excellently suited for trace determination of
one- to three valent ions of several transition metals. The formed complexes vary in composition and color
depending on pH and dipole strength of the solvent in use. Thus they can be applied for the discrimination of
different elements and their quantification by colorimetric.
Testsol is a proprietary product, which joins the advantages of
ecological harmlessness, lack of toxicity and inflammability under normal conditions, whereas the
chloromethanes used up to now are highly toxic and ecologically objectionable.
The comparative colorimetric evaluation is performed by matching color panels with printed colors
based on the Pantone code defined colors of pure complexes. In our hands this match was astonishingly
reliable.
We thoroughly investigated the sensitivity and specificity of
the HMST- system and we can furnish the proof at any time that the detection of the claimed individual metal
species of toxicological relevance is possible at the lower ppm- level. Under favorable circumstances we
could detect metal ions even at the ppb –level. Thus one can obtain important preliminary information for
clinical decisions in the medical cabinet or during a bed-side-examination.
The high sensitivity of this test-kit is naturally dependent on
the correct test-execution and prone to failure due to e.g. omitted pH-adjustments, shortened waiting periods
or wrong consecution of additions.
So you can see that there is no room for any doubts concerning
the respectability and reliability of the HMST Test-Kit. It is based on a scientifically approved method and
suitable for clinical purposes so that health insurance companies are supposed to refund the costs for its
execution.
Yours sincerely,
Dipl.-Chem.Dr.rer.nat. Joachim Leman
Further Excerpts from Expert Evaluations of Heavy Metal
Screening
[ ... ]
We thoroughly investigated the test system with regard to its sensitivity and
its specificity and found consistent evidence that it is possible to detect individual toxicologically relevant
metals in urine or water specimens in the range of a few ppm, at times even fractions of one ppm. This makes it
possible to obtain on the spot clinically important preliminary data [ ... ]
(J. Lemann, Dr. rer.nat., Toxicologist and Medical Expert, Institute for Toxicology and Medical Laboratory
Diagnostics, Hirschberg , Germany )
[ ... ]
This report is intended to be an independent assessment of the claims of
performance of a novel detection system for some transition metals. These materials are commonly known as toxic
or heavy metals. [...] it was found that the system shows
remarkable sensitivity for such a simple procedure [ ... ]
(K.H. Bell, Ph.D., Professor and Head, Department of Chemistry, The University of Newcastle,
NSW, Australia )
[ ... ] Until the beginning of the
nineteen-seventies this reagent was in predominant use for the detection of heavy metal traces in the water
supply. It is a certified German Government Standard procedure for water supply analyses. [ ...
]
(G. Schwedt, Dr. rer.nat., Professor and Director, Institute for Inorganic and
Analytical Chemistry, Technical University of Clausthal, Germany)
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