These findings indicate oxidative stress in patients with chronic renal failure which is
further exacerbated by hemodialysis and CAPD, as evidenced by increased lipid peroxidation ( MDA ) and
low antioxidant levels.
5. Ozden, Meltem; Akaydin, Derya; Cetinalp, Pinar; Kalender,
Betul; Maral, Hale JOURNAL NAME- Clinical Biochemistry VOL. 35 NO. 4 June, 2002 PP. 269-273. DOCUMENT TYPE- Article ISSN- 0009-9120
ADDRESS- E-Mail: mozden@superonline.com, Department of Clinical Biochemistry, Faculty of Medicine, Kocaeli
University of Kocaeli, Kocaeli , Turkey LANGUAGE- ENGLISH Objectives: Cardiovascular disease is
the major cause of mortality in patients receiving hemodialysis (HD) and continuous ambulatory peritoneal
dialysis (CAPD) due to chronic renal failure. Increased lipid peroxidation and depletion of antioxidants may
contribute to increased risk of atherosclerosis. We have therefore assessed the effect of hemodialysis and
CAPD on oxidant and antioxidant status. Design and methods: Plasma malondialdehyde ( MDA ), Glutathione (GSH)
levels and glutathione peroxidase (Gpx) activities were determined in 20 healthy persons (control), 20
patients on HD, 16 patients on CAPD. Results: MDA was elevated in posthemodialysis and CAPD patients in
comparison to prehemodialysis and control groups (posthemodialysis 1.39+-0.38 nmol/mL, CAPD 1.26+-0.27
nmol/mL, prehemodilaysis 0.83+-0.22 nmol/mL, controls 0.72+-0.21 nmol/mL p<0.0001). With respect to
antioxidants, glutathione levels were significantly lower in prehemodialysis, posthemodialysis and CAPD groups
than those in control group (prehemodialysis 16.82+-6.73 mg/dL RBC, posthemodialysis 31.43+-11.88 mg/dL RBC,
CAPD 40+-12.72 mg/dL RBC, controls 62.26+-24.01 mg/dL RBC, p<0.0001). While erythrocyte GSH levels were
significantly lower in the prehemodialysis patients than those in posthemodialysis and CAPD patients
(p<0.0001), it was significantly lower in posthemodialysis patients than those in CAPD patients
(p<0.05). There were no significant differences with respect to erythrocyte Gpx levels among the groups
(p>0.05). Conclusions: These findings indicate oxidative stress in patients with chronic renal failure
which is further exacerbated by hemodialysis and CAPD, as evidenced by increased lipid peroxidation and low
antioxidant levels.
Return from Free Radical Reference 5 MDA to Free
Radical Laboratory Validation 1.
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