In this study of the effect of ovariectomy, increases in
MDA make the determination that uterine oxidative stress is
increased.
4. Gomez-Zubeldia, M. A.; Arbues, J.; Corrales, S.; Millan,
J. C.; Nogales, A. G.
JOURNAL NAME- Gynecologic Oncology VOL. 86 NO. 3 September, 2002 PP. 250-258.
DOCUMENT TYPE- Article ISSN- 0090-8258 ADDRESS- School of Medicine, University of Extremadura , Avenida Elvas s/n., 06071, Badajoz , Spain
LANGUAGE- ENGLISH
Objective. We studied the effect of ovariectomy, estradiol (E2), and E2 + medroxyprogesterone (MPA) on the Wistar
rat uterus. Methods. We used 15 adult female rats. The study was divided into the following four stages: (a)
extirpation of the upper half of the left hemi-uterus (basal state) and ovariectomy; (b) animals were maintained
for 15 days without treatment, performance of a new laparotomy, and extirpation of the remaining left hemi-uterus
(OVX state); (c) beginning of E2 replacement therapy (ERT) (8 mug/day) for 15 days, followed by extirpation of the
upper half of the right hemi-uterus (ERT state); and (d) the administration of E2 was continued, and oral treatment
with MPA was begun (20 mug/day) to last for a further 15 days. At the end of the combined hormone replacement
therapy (HRT) the remaining right hemi-uterus was extirpated (HRT state). At the end of each intervention, the
plasma concentrations of E2 and PRG were measured. Results and Discussion. The ovariectomy significantly reduced
the malondialdehyde ( MDA ) levels (P < 0.0008) and catalase activity (P < 0.0006). The ERT very
significantly (P < 0.0033) raised the catalase and MDA levels ; these significance levels were maintained after
the Bonferroni method was applied (overall error 5%). The HRT reduced the levels of MDA and catalase, but not
significantly after the Bonferroni test was applied. Conclusions. Uterine oxidative stress is increased by E2,
resulting in a significant increase in MDA. This may be modulated in part by the catalase activity. Although it
cannot be confirmed categorically, MPA seems to intervene by decreasing the said oxidative stress.
Return from Free Radical Reference 4 MDA to Free Radical Laboratory
Validation 1.
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