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The free radical oxidative damage urine color test gives comparible results to laboratory tests in patients with Parkinson's. Laboratory validation follows.

URINE MALONDIALDEHYDE ( MDA ) MEASURED IN PARKINSON DISEASE, BY A FLUOROMETRIC AND A VISUALLY READ COLORIMETRIC ASSAY.

Parkinson's patients have a high amount of free radical malondialdehyde damage.

R. Hubbard, R. Iacono, J. Westengard, and Schoonenberg Depts. of Path., and Neurosurg. Sch. Med., Loma Linda Univ., Loma Linda, CA 92350.

We have measured the urine MDA levels in 17 Parkinson disease (PD patients and caregiver controls, by a fluorometric method (Conti et al., Clin. Chem. 37:1272,1991) and a one step visually read colorimetric method adapted from Miksch et al., Anal. Chem. 53:2118,1981, which is available through Vitaleaf Technologies, Irvine, CA. PD subjects and controls volunteered for the study while attending the 1999 Iocono Pallidotomy Reunion. They collected random midmorning urines and signed consent forms approved by the Investigative Review Board, Loma Linda University. To compare the two methods a Spearman rank order correlation coefficient 0.656 (p<0.0005) was computed. A one-way ANOVA with post hoc comparisons (Bonferroni) was performed to compare the mean fluorommetric MDA levels across the 4 semi-quantitative levels of the colorimetric method. The results showed that only the highest (level 3) compared to the lowest (level 0) were significantly different (p<0,02). Blind repetition of the colorimetric assay showed a perfect match in the reading for 27 of the 34 urine specimens. The seven non-matched results were within one level of matching. The difference in MDA levels between patients and controls was significant when measured by either the fluorometric (independent t-test, p=0.00005) or the colorimetric (Kruskal-Wallis, p=0.004) method.

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Free Radical Laboratory Validation 3

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