URINE MALONDIALDEHYDE ( MDA ) MEASURED BY A FLUOROMETRIC AND VISUALLY READ COLORIMETRIC ASSAY IN PARKINSON'S.    

This abstract represents a paper that was presented at the Federated Experimental Biology and Medicine meetings on April 15-18, 2000at San Diego, California .

R. Hubbard, R. Iacono, J. Westengard and T. Schoonenberg Depts. of Path., and Neurosurg. Sch. Med., Loma Linda Univ., Loma Linda, CA 92350.   

We have measured the urine Malondialdehyde ( MDA ) levels in 17 Parkinson disease (PD patients and care giver controls, by a fluorometric method (Conti et al., Clin. Chem. 37:1272, 1991) and a one step visually read colorimetric method adapted from Miksch et al., Anal Chem 53:2118, 1981, which is available through Vitaleaf Technologies, Irvine CA as OXISCORE. PD subjects and controls volunteered for the study while attending the 1999 Iocono Pallidotomy Reunion. They collected random midmorning urine's and signed consent forms approved by the Investigative Review Board, Loma Linda University . To compare the two methods a Spearman rank order correlation coefficient 0.656 (p<0.0005) was computed. A one-way ANOVA with post hoc comparisons (Bonferroni) was performed to compare the mean fluorometric MDA levels across the 4 semi-quantitative levels of the colorimetric method. The results showed that only the highest (level compared to the lowest (level 0) were significantly different (p<0.02). Blind repetition of the  colorimetric assay showed a perfect match in the reading for 27 of the 34 urine specimens. The seven non-matched results were within one level of matching. The difference in malondialdehyde levels between patients and controls was significant when measured by either the fluorometric (independent t-test, p=0.00005) or the colorimetric (Kruskal-Wallis, p=0.004) method. This abstract compares results determined with OXISCORE with a very complex and sensitive laboratory assay for the same molecule namely malondialdehyde (MDA). MDA is the first breakdown product from the action of "free radicals". We measure MDA in the urine because the MDA in the blood is quickly cleared from the body by the kidneys and the urine becomes a body fluid where the MDA is concentrated. The urine therefore is the ideal body fluid to measure MDA and thus a very sensitive way to look for free radical activity.  This study compares urinary MDA levels in Parkinson patients versus their normal mates or companion care givers. The two assay methods give very comparable results. OXISCORE showed that +3 results were significantly different from blank or zero values. Visual reading of the OXISCORE results read against the test comparison strip were very repeatable in a blind test comparison showed better than 85% correlation and no result was off by more than one level, e.g. +2 to a +3. The two assays gave very similar significantly higher MDA results in the Parkinson patients versus the controls with the complex laboratory assay giving somewhat more significant results. 

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